• 专利附录
  • 专利说明
  • 权利要求
  • 类似技术
  • 同族专利
  • 引用文献
  • 法律状态
  • 优先权
    • 专利摘要:
    To increase the sensitivity of the histological determinations, 19.6 to 27.3 wt.% Of the thionin dye is mixed with 38.2 to 41.5 wt.% Of resorcin, heated to 70-120 C. After cooling, 15.8-18.1 weight is added. % Alcian blue and 18.7-20.8 wt.% Safranin. W 00 sd and so cm
    公开号:SU1356971A3
    申请号:SU833625048
    申请日:1983-07-15
    公开日:1987-11-30
    发明作者:Балог Дьердь；Чаба Дьердь；Ковач Петер
    申请人:Реанал Финомведьсердьяр (Инопредприятие)；
    IPC主号:
    专利说明:

    eleven
    The invention relates to medicine, in particular, can be used for histological determinations.
    The purpose of the invention is to improve the quality of the composition to increase the sensitivity of the histological determinations.
    Example 1. 3.0 g of thionine and 1.3 g of resorcinol are dissolved in 1 l of water at room temperature (about
    ) and the solution is heated to boiling. After 3-5 seconds of boiling, the solution is cooled to 25 ° C, then 3.8 g of resorcinol is further added and the mixture is boiled for 3-5 seconds. Spreading out
    the thief is cooled to 25 ° C and 2.6 g of alcyan blue 8 GX gfi are added under agitation. The solution is filtered, the filtrate is brought to 1 liter with distilled water and 2.5 g of safranin are dissolved in the mixture. The resulting solution is again filtered and the volume of the filtrate is adjusted to 1 liter with distilled water.
    The solution can be used to dye the fabric.
    If the composition of the dye needs to be stored for a long period, it is subjected to vacuum overflow or freeze-drying. It is possible to obtain 12 from the polished solution, the composition of the dye in the form of a powder.
    The dye is used for histological determinations as follows.
    A fresh slice, obtained, for example, by surgery, is frozen, and then stained for 10 minutes with a solution of the composition. The section is washed with tap water for no longer than 1 minute, promacit with tissue paper and then treated maximum twice with tertiary butanol for 20 seconds (cf. dehydration). Then, the section was clarified twice with xylene for 1 minute each time and coated with Canadian balsam. Thus, after 25-30 minutes, a section is prepared for histological studies.
    The various components of the fabric are colored in the following colors: the active cores are blue-green; passive cores, cores before or after decomposition (the amount of which is significant in tumor tissues) are red; conjunctive tissue syn; smooth mu








    cool - pink sh; mucinna gland - syn; iron cirrhosis is red; mitochondri - violet grains; hr y - purple, purple, pink or green-blue, depending on maturity; neurosecretory grains are red.
    Example 2, the work is carried out
    logical for example 1, use 3.3 g of toluidine blue instead of thionin, as well as 4.6 g of resorcinol. The mixture of toluidine blue and resorcinol is heated to 70 ° C, after which 1.9 g of alcian blue and 2.25 g of safranin are added to the cooled solution. Get results similar to the results of example 1.
    Example 3. Work is carried out analogously to example 1, using 2.6 g of azure A instead of thionin, as well as 5.5 g of resorcinol,. A mixture of azure A and resorcinol is heated at an overpressure of up to .120 ° C. To chilled
    2.4 g of alcyan blue and 2.75 g of safranin are added to the solution. Get results similar; with the results of example 1.
    All used; weights have
    The degree of purity of the analytical grade is the following: molecular weight:
    five
    Thionin
    Toluidine
    blue
    Azur A
    Alcian
    blue
    Resorcin
    Safranin
    263.75
    305.83 291.80
    1300 110.11 350.85
    40
    45
    权利要求:
    Claims (1)
    [1]
    Invention Formula
    The method of obtaining the composition for histological studies by using safranin, alcyan blue, resorcinol, and thionin dye as dyes, which is a matter of fact, in order to improve the quality of the composition for
    5Q increase in the sensitivity of histological determinations, pre-mix 19.6-27.3 wt.% Of thionin dye with 38.2-41.5 wt.% Of resorcin, the mixture is heated to
    55 70-120 ° C, cooled, after which 15.8-18.1 wt.% Alcian blue and 18.7-20.8 wt.% Safranin are added to it.
    类似技术:
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    同族专利:
    公开号 | 公开日
    FI74036B|1987-08-31|
    NO832557L|1983-07-13|
    WO1983001779A1|1983-05-26|
    JPS58501968A|1983-11-17|
    RO88189B1|1985-12-31|
    CS830782A2|1988-09-16|
    AT23180T|1986-11-15|
    FI832357A0|1983-06-28|
    DE94398T1|1985-04-25|
    DK333283D0|1983-07-19|
    US4595582A|1986-06-17|
    EP0094398B1|1986-10-29|
    CU21441A3|1987-01-13|
    DK333283A|1983-07-19|
    HU184903B|1984-11-28|
    DE3273990D1|1986-12-04|
    FI832357L|1983-06-28|
    EP0094398A1|1983-11-23|
    RO88189A2|1985-12-30|
    DD268844A7|1989-06-14|
    EP0094398A4|1984-04-06|
    FI74036C|1987-12-10|
    AU9126882A|1983-06-01|
    引用文献:
    公开号 | 申请日 | 公开日 | 申请人 | 专利标题
    US3440317A|1964-11-09|1969-04-22|Arfilio J Martinez|Cell coloring process and composition for cytological examination|
    GB1293757A|1968-10-22|1972-10-25|Paines & Byrne Ltd|A nerve stain composition|
    GB1272025A|1970-04-22|1972-04-26|Institutual Pentru Controlul D|Method for determining cellular ribonucleic acid distribution|
    US3906120A|1970-10-30|1975-09-16|Gen Electric|Method for preparing slides for blood evaluation|
    US3916205A|1973-05-31|1975-10-28|Block Engineering|Differential counting of leukocytes and other cells|
    US4146604A|1973-05-31|1979-03-27|Block Engineering, Inc.|Differential counting of leukocytes and other cells|
    US3928554A|1973-12-19|1975-12-23|Block Engineering|Emulsion dyeing|
    FR2278739B1|1974-07-19|1976-12-24|Ugine Kuhlmann|
    DE2828227A1|1978-06-28|1980-01-10|Bayer Ag|PHTHALOCYANIN REACTIVE DYES|
    US4382075A|1981-04-03|1983-05-03|Miles Laboratories, Inc.|Stabilized Romanowsky stain solution|
    HU184903B|1981-11-20|1984-11-28|Reanal Finomvegyszergyar|Paint composition for biopsy examinations|HU184903B|1981-11-20|1984-11-28|Reanal Finomvegyszergyar|Paint composition for biopsy examinations|
    DE3533515A1|1985-09-20|1987-04-02|Merck Patent Gmbh|STABILIZER FOR COLOR SOLUTIONS|
    US5281517A|1985-11-04|1994-01-25|Cell Analysis Systems, Inc.|Methods for immunoploidy analysis|
    US5202931A|1987-10-06|1993-04-13|Cell Analysis Systems, Inc.|Methods and apparatus for the quantitation of nuclear protein|
    US5134662A|1985-11-04|1992-07-28|Cell Analysis Systems, Inc.|Dual color camera microscope and methodology for cell staining and analysis|
    US5109429A|1985-11-04|1992-04-28|Cell Analysis Systems,Inc.|Apparatus and method for analyses of biological specimens|
    EP0294529B1|1987-06-12|1994-08-31|HYMAN, Edward S.|Determination of bacteriuria|
    FR2639714B1|1988-11-30|1991-02-15|Oreal|DIFFERENTIAL COLORING PROCESS FOR SKIN CELLS AND THEIR CORES|
    AT175431T|1989-09-20|1999-01-15|Silberline Mfg Co|COLORED METAL PIGMENTS|
    EP0445434B1|1990-03-07|1997-11-05|Cytyc Corporation|Thionin stain technique|
    US20020058028A1|1999-05-05|2002-05-16|Mark K. Malmros|Method of in situ diagnosis by spectroscopic analysis of biological stain compositions|
    GB2355790A|1999-10-27|2001-05-02|Kay Cottington|Detecting cancer|
    US6593102B2|1999-10-29|2003-07-15|Cytyc Corporation|Cytological stain composition|
    AU1242101A|1999-10-29|2001-05-14|Cytyc Corporation|Cytological stain composition|
    US6348325B1|1999-10-29|2002-02-19|Cytyc Corporation|Cytological stain composition|
    US7771189B2|2008-03-03|2010-08-10|R&D Tool & Engineering Co.|Injection molding apparatus with replaceable gate insert|
    法律状态:
    优先权:
    申请号 | 申请日 | 专利标题
    HU813471A|HU184903B|1981-11-20|1981-11-20|Paint composition for biopsy examinations|
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